Fig. 8.

Connections to regulators of osteogenesis and cellular proliferation exhibited by proteins identified by 2D-DIGE. This network, generated by Ingenuity®, portrays relationships described in the literature between all of the proteins identified by 2D-DIGE. Runx2 was added to emphasize the participation of these proteins in the osteogenic pathway. The proteins from the gel spots are colored in varying shades of red relative to the match score they received from Mascot. Together with TGF-β1, Runx2 and TP53 are hubs for regulation of osteogenesis and cellular proliferation. (Note: Gray arrows indicate action, not up/down-regulation. Only lines leading to and away from the four focus genes are color-coded.) Protein symbols are listed in (Additional file 8: Table S2). 2D-DIGE 2D-differential in-gel electrophoresis