Table 2.
Identities of Proteins from "Pick List"
| Symbol | Entrez Gene Name | Found in Spots: | Mx | Max Score | pI | Mr | ||
|---|---|---|---|---|---|---|---|---|
| CYTOPLASM | ACTA2 | actin, alpha 2, smooth muscle, aorta | 826, 894–6, 1053 | W | 348 | 5.23 | 42,381 | |
| ALDH1B1 | aldehyde dehydrogenase 1 family, member B1 | 658 | W | 283 | 6.36 | 57,658 | ||
| AKR1C1 | aldo-keto reductase family 1, member C1 | 894 | W | 240 | 8.02 | 37,221 | ||
| ATP5A1 | ATP synthase, H+ transport, mitoch F1 complex, alpha 1 | 658 | W | 867 | 9.16 | 59,828 | ||
| ATP5B | ATP synthase, H+ transport, mitoch F1 complex, beta | 689 | W | 683 | 5.26 | 56,525 | ||
| CKAP4 | cytoskeleton-associated protein 4 | 717 | W | 821 | 5.63 | 66,097 | ||
| ENO1 | enolase 1, alpha | 758, 759 | W | 532 | 7.01 | 47,481 | ||
| ENO3 | enolase 3, beta, muscle | 758 | W | 246 | 7.59 | 47,299 | ||
| EIF3B | eukaryotic translation initiation factor 3, subunit B | 896 | W | 213 | 5.38 | 36,878 | ||
| FH | fumarate hydratase | 758, 759 | W | 290 | 8.85 | 54,773 | ||
| HSP90B1 | heat schock protein 90kDa beta (Grp94), member 1 | 449, 636, 637 | B | 306 | 4.76 | 92,696 | ||
| HSPB1 | heat shock 27kDa protein 1 | 1053 | W | 315 | 5.98 | 22,826 | ||
| HSPD1 | heat shock 60kDa protein 1 (chaperonin) | 607 | W | 390 | 5.83 | 60,813 | ||
| HSPA5 | heat shock 70kDa protein 5 (glucose-regulated) | 449, 486 | B | 1483 | 5.07 | 72,402 | ||
| IKIP | IKK interacting protein | 717 | W | 487 | 9.21 | 39,399 | ||
| PRDX1 | peroxiredoxin 1 | 1093 | W | 363 | 8.27 | 22,324 | ||
| P4HB | prolyl 4-hydroxylase, beta polypeptide | 201, 607, 636 | B | 1116 | 4.76 | 57,480 | ||
| PSMD13 | proteasome (prosome, macropain) 26S subunit, 13 | 826 | W | 219 | 5.71 | 42,872 | ||
| PDIA3 | protein disulfide isomerase family A, member 3 | 647-9 | W | 706 | 5.98 | 57,146 | ||
| PDIA6 | protein disulfide isomerase family A, member 6 | 689 | W | 395 | 5.35 | 48,207 | ||
| PDHB | pyruvate dehydrogenase (lipoamide) beta | 896 | W | 202 | 6.20 | 39,550 | ||
| PKM2 | pyruvate kinase, muscle | 607 | W | 341 | 7.96 | 58,470 | ||
| SOD2 | superoxide dismutase 2, mitochondrial | 1093-6 | W | 347 | 8.35 | 24,878 | ||
| TUBA1A | tubulin, alpha 1A | 689, 894 | W | 494 | 4.94 | 50,788 | ||
| ECM | COL6A1 | collagen, type VI, alpha 1 | 201, 203, 208, 210, 224, 242, 247 | D | 1432 | 5.26 | 109,602 | |
| COL6A3 | collagen, type VI, alpha 3 | 247, 607, 649 | B | 321 | 6.26 | 345,163 | ||
| EFEMP2 | EGF-containing fibulin-like ECM protein 2 | 636 | W | 297 | 4.79 | 51,725 | ||
| EMILIN1 | elastin microfibril interfacer 1 | 224-6, 242, 247 | D | 462 | 5.07 | 107,913 | ||
| HTRA1 | HtrA serine peptidase 1 | 637 | W | 267 | 8.09 | 52,167 | ||
| TGFBI | transforming growth factor, beta-induced, 68kDa | 486 | W | 247 | 7.62 | 75,261 | ||
| PM | ANXA1 | annexin A1 | 894-6 | W | 397 | 6.57 | 38,918 | |
| ANXA2 | annexin A2 | 894-6 | W | 1201 | 8.44 | 36,950 | ||
| ANXA6 | annexin A6 | 486 | W | 265 | 5.42 | 76,168 | ||
| FN1 | fibronectin 1 | all but 1093-96 | D | 2242 | 5.45 | 266,034 | ||
| FLOT1 | flotillin 1 | 758 | W | 496 | 7.08 | 47,554 | ||
| NU | CRYAB | crystallin, alpha B | 1093-6 | W | 244 | 6.76 | 20,146 | |
| LMNA | lamin A/C | 561-66 | W | 1179 | 6.57 | 74,380 | ||
Columns show (left to right): the number(s) of the spot(s) where a given protein was found; the type of matrix in which the protein was more abundant (D = DOC, W = water, B = both); maximum Mascot identity score from mass spectrometry; and theoretical pIs and Mr values determined by Mascot from the protein sequences. Bolded text indicates that the approximate pI and/or Mr of the spot in which the protein was found match its theoretical values. Proteins are grouped by subcellular location based on Ingenuity’s database
NU nucleus, ECM extracellular matrix, PM plasma membrane