Fig. 8.
In vitro time-domain optical coherence tomography (TD-OCT) measurement of frog (a) and rabbit (b) retinas. (a1) A voltage pulse of 10 ms was used to drive a white light-emitting diode (LED), and the light flash stimulated the frog retina. (a2) Electrophysiological response associated with the light stimulus. (a3) Scattering response at the photoreceptor layer. (a4) Scattering response at the ganglion layer. Each trace is an average of 100 trials, and the recording interval was 1.5 s. (b1, left) A morphological retinal image compared with differential M-scans corresponding to a dark scan (no light stimulus), (middle) a single flash scan in normal retina, and (right) a single flash scan in retina with inhibited bipolar cell function in the inner plexiform layer (IPL). The red dashed line marks the boundaries of the IPL in all M-scans. (b2, b3) A 3-D visualization of the positive and negative optical signals in different M-scans. (b4) The time course of the optical signals (average across the width of the IPL) extracted from the IPL. The error bars show the standard deviation computed by averaging 10 differential M-scans. (b5) The ERG recordings simultaneously acquired with the OCT M-scans. (b1–b3) The white and (b4,b5) yellow strips in the M-scans mark the onset and time duration of the SF light stimulus. (a) Reprinted with permission from reference 68. (b) Reprinted with permission from Ref. 114.