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. 2015 Dec 14;212(13):2289–2304. doi: 10.1084/jem.20150388

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© 2015 Phong et al.

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Figure 2. — Tim-3 regulates cytokine production by BMMCs. (A) BMMCs from WT or Tim-3 KO mice were differentiated as described in Materials and methods. Maturity of BMMCs was determined by FcεRI and c-kit staining. Tim-3 surface expression was compared between WT and Tim-3 KO BMMCs, which were sensitized with IgE overnight and stimulated with the indicated amount of Ag and Tim-3 pAb or 5D12 mAb. (B-C) Supernatants were collected after 6 h and analyzed by ELISA for IL-6. (B) BMMCs were stimulated with low valency (DNP₅; left) or high valency (DNP₃₂; right) Ag. (C) BMMCs were stimulated with high valency Ag, plus isotype control, Tim-3 pAb (left), or Tim-3 mAb (right). (D) BMMCs were stimulated with high valency Ag alone (left), or with isotype control or Tim-3 pAb (right), followed by quantitation of degranulation by flow cytometry. Results are representative of three independent experiments performed in duplicate. *, P < 0.05; **, P < 0.005.