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. 2015 Dec 14;212(13):2289–2304. doi: 10.1084/jem.20150388

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© 2015 Phong et al.

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Figure 3. — Tim-3 knockdown attenuates Ag-induced cytokine production by BMMC. BMMCs generated from C57BL/6 mice were transfected with control (nonsp) or Tim3-specific siRNA. (A) 48 h later, cells were analyzed by flow cytometry for Tim-3 expression. (B and C) BMMCs were sensitized overnight with IgE and stimulated with DNP₃₂-HSA for 6 h. IL-6 secretion was assessed by ELISA, using either Tim-3 pAb (B) or 5D12 (C) for co-stimulation. Results are representative of three independent experiments, performed in duplicate. *, P < 0.05; **, P < 0.005; ***, P < 0.0005.