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. 2015 Dec 14;212(13):2289–2304. doi: 10.1084/jem.20150388

Figure 7.

Figure 7.

Tim-3 cross-linking enhances PLC-γ1 and ribosomal protein S6 phosphorylation. (A) BMMCs were sensitized with IgE overnight and stimulated as indicated for 10 min, lysed, and analyzed by Western blotting for phospho-PLC-γ1 (top), total PLC-γ1 (middle), and actin. (right) Quantification of relative proportions of PLC-γ1 phosphorylation. (B and C) Nur77GFP BMMCs were stimulated as indicated and analyzed for pS6 (S235/236) by flow cytometry. Results are quantitated as %pS6hi (B) and %pS6+GFP+ cells (C). Results are representative of three independent experiments. *, P < 0.05.