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. 2015 Dec 14;212(13):2289–2304. doi: 10.1084/jem.20150388

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© 2015 Phong et al.

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Figure 8. — Tim-3 regulates FcεRI positive versus negative signaling. (A). PCA responses in WT (n = 13) and Tim-3 KO (n = 11) mice were measured as described in the Materials and methods. Results are representative of three independent experiments totaling 19 WT and 14 Tim-3 KO mice. Outliers were removed using QuickCalcs software (GraphPad). Lyn siRNA-treated cells were sensitized with IgE and stimulated with low valency (B) or high valency (C) Ag, plus isotype control, Tim-3 pAb, or Tim-3 mAb. (D) Efficiency of siRNA-mediated Lyn knockdown in BMMCs. Results shown are representative of three independent experiments performed in duplicate. (E) Proposed model for the effects of Tim-3 engagement or deficiency on the functional outputs downstream of FcεRI signaling. Note that the effects of Tim-3 deficiency closely mirror those of partial loss-of-function of Lyn kinase activity.