Skip to main content
. 2015 Dec 14;212(13):2213–2222. doi: 10.1084/jem.20151250

Figure 1.

Figure 1.

FDCs are required for P2RY8-dependent confinement to the center of the follicle. (A–E) Activated Gpr183+/− B cells (see Materials and methods) were transduced with retroviral vector encoding Thy1.1 alone (vector) or P2RY8 and Thy1.1 and transferred into WT animals previously immunized with sheep red blood cells (SRBC; A), CD19-deficient animals lacking splenic GCs (B), WT animals treated with LTβR-Fc or human IgG for 1 wk (C), CD21-DTR or littermate control reverse BM chimeras treated with DTx at the time of transfer (D), or previously immunized Cxcl13−/− animals (E, arrows indicate ectopic FDC; two images are shown for each condition). Spleens were harvested 1 d after transfer and analyzed immunohistochemically for the presence of transduced cells (Thy1.1), FDCs (CD35), GC B cells (GL7), or naive follicular B cells (IgD) as indicated. Bars, 100 µm. Images are representative of four, two, two, two, and three independent experiments, respectively. (F) qPCR of S1PR2, P2RY8, and BCL6 from CXCR5− (non-Tfh), CXCR5 intermediate (pre-GC Tfh), and CXCR5-high (GC Tfh) CD4+ T cells from human tonsil. Data are from four donors and lines indicate means. **, P < 0.01; ***, P < 0.001, unpaired two-tailed Student’s t test.