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. Author manuscript; available in PMC: 2016 Sep 14.
Published in final edited form as: Biomacromolecules. 2015 Aug 17;16(9):2684–2692. doi: 10.1021/acs.biomac.5b00557

Figure 3.

Figure 3

Proliferation studies of different sizes of pVS polymers on BaF3-FR1C cells. The cells were incubated in the presence of 1 ng/mL bFGF and various concentrations of heparin, pVS 6.6 kDa, 14.4 kDa, 24.6 kDa, 35.9 kDa, and 80.3 kDa. Incubation of 20,000 cells/well in 96-well plate with each of the samples was carried out for 48 hours. CellTiter®-Blue assay was performed to quantify the extent of cell growth. Data was normalized to the blank medium group, which was set at 100%. Each sample had four replicates and the experiment was repeated three times. Error bars represent SEM. Statistical analysis was performed using Student’s t-test; p < 0.05 was consider significant. The percent cell growth from each polymer was compared to the control group, which had heparin at the same concentration. No statistical difference was found between the pVS polymers and heparin at all concentrations tested.