Skip to main content
. Author manuscript; available in PMC: 2016 Sep 23.
Published in final edited form as: J Am Chem Soc. 2015 Sep 15;137(37):12100–12109. doi: 10.1021/jacs.5b07772

Figure 1.

Figure 1

Role of ArCP in yersiniabactin synthesis. a) ArCP is the first carrier protein domain of HMWP2, which also comprises two cyclization domains (Cy1 and Cy2), two peptidyl carrier proteins (PCP1 and PCP2), an adenylation domain for cysteine (A), and an epimerization domain embedded in the A-domain (E). b) The A-domain loads cysteine onto PCP1 and PCP2, and the stand alone A-domain YbtE loads salicylate onto ArCP. c) Cy1 catalyzes the condensation and cyclodehydration of Sal and Cys, forming 2-hydroxyphenylthiazoline on PCP1 and returning ArCP to its holo form. Not shown: Cy2, HMWP1 (a mixed PKS NRPS protein), and YbtU complete the synthesis of yersiniabactin (e). d) Nomenclature used to assign phosphopantetheine (J-U) and salicylate (V-AA) is shown in italics.