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. 2015 Dec 15;142(24):4363–4373. doi: 10.1242/dev.113746

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© 2015. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 2. — Analysis of brain vascular pathologies in mice lacking Nrp1 in endothelial cells or β8 integrin in neuroepithelial cells. (A-D) Horizontal sections through the ganglionic eminences of control (A), Alk1-Cre;Nrp1^fl/fl (B), Nestin-Cre;β8^fl/fl (C), or β8^−/− (D) embryos labeled with Isolectin B4-Alexa Fluor 488 to reveal blood vessels. Lower panels are digitally magnified images of boxed areas in upper panels. Note the abnormal blood vessel patterning in the mutant brains (arrows). (E) Horizontal sections through the thalamus of control (left), Alk1-Cre;Nrp1^fl/fl (middle) and β8^−/− (right) E13.5 brains were labeled with anti-CD31 antibodies. Shown are representative three-dimensional reconstructions of the brain vasculature. At this developmental age, note that Nrp1 mutant blood vessels fail to sprout normally and do not reach the subventricular zone (dashed line), whereas blood vessels in the β8^−/− brain display abnormal hyper sprouting near subventricular regions.