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. 2015 Dec 15;142(24):4217–4229. doi: 10.1242/dev.123612

Fig. 2.

Fig. 2.

notum and wnt11-6 are expressed in neurons at opposite poles of the brain. (A) Double FISH to detect notum (cyan), wnt11-6 (magenta) and chat (gray) expression in uninjured animals. notum is expressed at the anterior pole and in the brain commissure, whereas wnt11-6 is expressed at the posterior of each brain lobe (arrows). (B,C) Double FISH detecting expression of notum and either collagen (B) or chat (C). Of the notum+ cells at the anterior pole, 85.5±4.6% express collagen, marking musculature (out of 131 cells counted in five animals), whereas 90.1±4.4% of notum+ cells at the anterior commissure express chat, marking neurons (out of 81 notum+ cells counted in five animals; white arrows, double positive cells; yellow arrows, cells that express only notum). (D) Of the wnt11-6+ cells near the posterior brain, 51.3±0.7% express chat (out of 2362 wnt11-6+ cells counted in four animals). (E) Double FISH detecting expression of notum (cyan) and wnt11-6 (magenta) in head fragments undergoing brain remodeling (top panels) and trunk fragments forming a new brain through epimorphosis (bottom panels). Scale bars: 50 µm in A-D; 150 µm in E. Anterior, top. d, day.