Fig. 7.

notum and wnt11-6 regulate numbers of brain cell progenitors. (A,B) Double FISH to detect coexpression of smedwi-1 (green) and ap2, lhx1/5-1 or coe (magenta) in (A) trunk fragments regenerating a new brain or (B) head fragments undergoing brain remodeling 4 days after amputation and treated with indicated dsRNAs. Upper panels, images are representative planes from a confocal stack of the region indicated by the cartoon (white arrows, double positive cells; yellow insets show magnified representative double positive cells). Lower panels, graphs show quantifications of progenitor cell numbers (see Materials and Methods for details; n=4 animals per condition). Error bars indicate s.d. *P<0.05, **P<0.005, n.s. P>0.05, two-tailed t-test. Scale bars: 100 µm. Anterior, left in A; top in B.