Fig. 2.

Effects of bromelain on the levels of iNOS production by LPS-stimulated primary microglial cells. Primary microglial cells were pretreated with bromelain at 5-30 µg/ml for one hour before LPS (1 µg/ml) addition. (A) After incubation for 48 hours, the expression of iNOS mRNA levels was measured by semi-quantitative RT-PCR analysis. The results showed that the LPS-stimulated increase of iNOS levels in the primary microglial cells was reduced to the control levels in the presence of 30 µg/ml bromelain. LPS and bromelain treatments are shown with L and B, respectively in the Figure. (B) Densitometry analysis of the bands was performed by Totallab software. *P<0.01 as compared with the LPS-treated group without bromelain