Fig. 3.

Effects of bromelain on the levels of NF-κB p65 production by LPS-stimulated primary microglial cells. Primary microglial cells were pretreated with bromelain at 30 µg/ml for one hour before LPS (1 µg/ml) addition. (A) After incubation for 48 hours, the expression of NF-κB p65 protein levels was measured by semi-quantitative Western-blot analysis. The results showed that the LPS-stimulated increase of NF-κB levels in the primary microglial cells was reduced to the control levels in the presence of 30 µg/ml bromelain. LPS and bromelain treatments are shown with L and B respectively in the picture. (B) Densitometry analysis of the bands was performed by Totallab. *P<0.01 as compared with the LPS-treated group without bromelain