Figure 2.

Oxytocin receptor signaling is dispensable for non-social learning. (A) Schematic of the odor-driven non-social appetitive learning paradigm. For water-restricted animals, 10% sucrose solution was used as a reward. For animals fed ad lib, palatable food was used as a reward. During training, presentation of the CS+ odor (purple shade) was paired with reward presented on a wire cage. The CS− odor (orange shade) was paired with an empty wire cage. For testing, the CS+ or the CS− odor was presented without reward when the subject entered the randomly pre-determined CS+/CS− chamber. (B) Time spent in each chamber of saline (S–S) or OTA (OTA-OTA)-injected mice in the sucrose-reward scheme. **: P < 0.01 by Tukey HSD post hoc test. (C) Preference score for data presented in (B). P = 0.84 by Student’s t-test. (D) Time spent in each chamber of S-S or OTA-OTA mice in the food-reward scheme. **: P < 0.01 by Tukey HSD post hoc test. (E) Preference score for data presented in (D). P = 0.39 by Student’s t-test. (F) Schematic of the aversive non-social learning paradigm. During training, the CS+ odor was presented on the side where the subject was located, followed by application of a mild foot shock. During testing, the CS+ odor was presented in the absence of shock. (G) Percentage of flight behavior displayed by S-S or OTA-OTA mice in response to the CS+ presentation. P = 0.33 by Student’s t-test. Data presented as mean ± SEM.