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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: J Bone Miner Res. 2015 May;30(5):837–854. doi: 10.1002/jbmr.2421

Fig. 1.

Fig. 1

Netrin-1 and Unc5b are expressed during osteoclast but not osteoblast differentiation. (A) Total RNA was extracted from both undifferentiated and osteoclast-derived RAW264.7 cells, and quantitative RT-PCR analysis of the Netrin-1 guidance cue members was performed using RT2 Custom Profiler PCR Arrays. Fold change in expression is shown. (B) Fold change in Netrin-1, Unc5b, and DCC mRNA in osteogenic differentiated osteoblast after 10 days of osteoblast differentiaion in murine BMCs. (C) Fold change in Netrin-1, Unc5b, and DCC mRNA in murine M-CSF/RANKL osteoclast precursors during the 7 days of osteoclast differentiaion. ***p < 0.001, **p < 0.01, versus nonstimulated control (Student’s t test).