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Unc5b modulates the expression of RGMa in osteoclast precursors. RAW264.7 cells were stably transduced with scrambled or Netrin-1, Unc5b, or DC-STAMP shRNA and treated with 50 ng/mL RANKL for 24 hours. RGMa immunostaining is shown in green and Unc5b immunolocalization is shown in red. Images were taken at an original magnification of ×63.
