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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: J Bone Miner Res. 2015 May;30(5):837–854. doi: 10.1002/jbmr.2421

Fig. 8.

Fig. 8

Stimulation of Unc5b promotes cell fusion associated with DC-STAMP. (A) Murine BMCs were treated with 30 ng/mL RANKL together with recombinant Netrin-1. Cell extracts were immunoprecipitated with anti-Unc5b antibody. The immunoprecipitates were then analyzed by immunoblotting with anti-DC-STAMP antibody. The figure shows representative data from one of four experiments. (B) RAW264.7 cells were stably transduced with scrambled or Netrin-1, Unc5b, or DC-STAMP shRNA and treated with 50 ng/mL RANKL for 4 days. DC-STAMP immunostaining is shown in green and Unc5b immunolocalization is shown in red. Images were taken at an original magnification of ×63.