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. 2016 Jan;57(1):89–99. doi: 10.1194/jlr.M064121

Fig. 3.

Fig. 3.

Conversion of ARA from DGLA by D6d desaturase mutant D6d-Z2 carrying 12 D5d amino acids. Intact D6d (B), intact D5d (C), and D6d-Z2 (D) were expressed in yeast in presence of DGLA (20:3 Δ8,11,14; peak 5), and the generation of ARA (20:4 Δ5,8,11,14; peak 6) was detected by GC. Yeast harboring the pYES2 vector was used as negative control (A). Other peaks in A–D are 16:0 (peak 1), 16:1 Δ9 (peak 2), 18:0 (peak 3), and 18:1 Δ9 (peak 4). DMOX-derivative of ARA generated by the mutant D6d-Z2 (peak 6 in D) was identified by GC/MS analysis (E) as described in the Materials and Methods. Predicted molecular mass numbers of daughter ions of ARA are shown on the structural formula.