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Conversion of ARA from DGLA by desaturase mutants with point mutation(s). A: D6d-F322L. B: D6d-L323F. C: D6d-I326V. D: D6d-E413K. E: D6d-L323F-I326V. F: D6d-L323F-I326V-E413K. G: D6d-25m. H: Intact D6d. Each mutant was expressed in yeast in the presence of DGLA (peak 1), and the generation of ARA (peak 2) was detected by GC.
