TABLE 3.

D5d and D6d activities of D6d mutants carrying zD5/6d-type amino acids

Substitution	1C-1	1C-2	1C-3	2C-1	2C-2	2C-3	2C-4	3C-1	3C-2	3C-3	4C-1	4C-2	4C-3	4C-4	5C-1	D6d-25m
N156T	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+
A305V	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+
Y182F		+	+	+	+	+	+	+	+	+	+	+	+	+	+	+
K234N		+	+	+	+	+	+	+	+	+	+	+	+	+	+	+
S235M		+	+	+	+	+	+	+	+	+	+	+	+	+	+	+
E365K			+	+	+	+	+	+	+	+	+	+	+	+	+	+
F166V				+	+	+	+	+	+	+	+	+	+	+	+	+
L423F				+		+	+	+	+	+	+	+	+	+	+	+
L424A				+		+	+	+	+	+	+	+	+	+	+	+
L248V				+			+	+	+	+	+	+	+	+	+	+
F322L					+	+	+	+	+	+	+	+	+	+	+	+
L323F					+	+	+	+	+	+	+	+	+	+	+	+
I195L							+	+	+	+	+	+	+	+	+	+
W245V							+	+	+	+	+	+	+	+	+	+
L396Y								+	+	+	+	+	+	+	+	+
Y257H								+		+	+	+	+	+	+	+
V344P									+	+	+	+	+	+	+	+
I284F										+	+	+	+	+	+	+
F370A											+	+		+	+	+
Y352N											+		+	+	+	+
I326V												+	+	+	+	+
Y188F														+	+	+
K190T														+	+	+
H410Y													+		+	+
E413K																+
D5d activity (%)	0	0	0	0	2.5	2.6	1.3	2.4	2.8	2.6	3.4	6.2	4.4	4.8	5.3	6.8
D6d activity (%)	16.3	17.4	13.7	5.3	11.6	10.0	13.5	13.0	13.0	16.0	13.8	11.0	21.3	21.3	26.8	26.3

D5d and D6d activities were represented as conversion rate from substrates (18:2 Δ9,12 for D6d and 20:3 Δ8,11,14 for D5d) to products (18:3 Δ6,9,12 for D6d and 20:4 Δ5,8,11,14 for D5d, respectively). The plus sign (+) indicates mutation points of each D6d mutant carrying zD5/6d-type amino acids.