Fig. 5.

Hematopoietic ABCA1 deletion led to leukocytosis and monocytosis with HFHSC diet feeding. Analysis of peripheral blood by hematology cell counter and flow cytometry of BMT-WT and BMT-ABCA1^−/− mice fed a chow or HFHSC diet for 21 weeks. A: White blood cell counts. MO, monocytes; NE, neutrophils; EO, eosinophils; LY, lymphocytes. B: Flow cytometry gating strategy for blood monocytes and subsets and representative histograms. C: Total monocytes were defined as CD115^hi, divided into Ly6c^lo and Ly6c^hi subsets and quantified. D: Representative histogram of myeloid cells in peripheral blood. E: Myeloid cells were defined as CD11b^hi and Ly6c^hi and quantified in peripheral blood. Results are means ± SD of five mice per group. *P < 0.05 versus BMT-WT; #P < 0.05 HFHSC diet effect.