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. 2015 Dec 23;10(12):e0145887. doi: 10.1371/journal.pone.0145887

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© 2015 Wang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 2 — A. The predicted mRNA secondary structure and minimal free energy for the indicated signal sequences. B. The expression and secretion levels of firefly luciferase with the indicated signal peptide. Intracellular and extracellular luciferase activities were separately detected, and the sum of the two was used to calculate the relative enzyme activity. **p<0.01 vs. the wild type signal sequence.