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. 2015 Dec 23;10(12):e0145883. doi: 10.1371/journal.pone.0145883

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© 2015 Jamet et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 1 — (A) Genetic organization showing the replacement of the hadC gene and hadABC operon by a zeocin resistance cassette (Sh ble) and the primers a, b, c, d used for PCR verification of the constructions. (b+c) primer couple confirms the deletion of the hadC gene (B) and hadABC operon (D). Amplification with the (b+d) and (a+d) primer couples confirms the presence of the zeocin cassette in the ΔhadC (C) and ΔhadABC (E) strains, respectively. PCR was performed on cell lysates: wt (lane 1), ΔhadC (lane 2), ΔhadABC (lanes 3–6) carrying either the plasmid pABC (lane 3) or pAB (lane 4) or pBC (lane 5) or pB (lane 6).