Table 1. List of strains and plasmids.

Bacterial strains	description	sources
mc2 155	M. smegmatis mc 2 strain	[26]
ΔhadC	native copy of MSMEG_1342 replaced by Sh ble (ZeoR)	This work
ΔhadABC pABC	native copy of MSMEG_1340/1341/1342 operon replaced by Sh ble (ZeoR), and carrying the pABC plasmid	This work
MC1061 recA	E. coli cloning strain	Our collection
Plasmids
pJV53	KanR, Plasmid expressing the recombineering proteins	[27]
pJV53::hyg	HygR, Hygromycin resistant derivative of Plasmid pJV53	V. Malaga
pGBT	KanR, C. glutamicum tetRO promoter cloned in pGB9.2	[28]
pMVZ261	ZeoR, source of the Sh ble gene	G. Etienne
pC	KanR, native copy of MSMEG_1342 cloned in pGBT	This work
pABC	KanR, native copy of MSMEG_1340/1341/1342 in pGBT	This work
pAB	KanR, native copy of MSMEG_1340/1341 cloned in pGBT	This work
pBC	KanR, native copy of MSMEG_1341/1342 cloned in pGBT	This work
pB	KanR, native copy of MSMEG_1341 cloned in pGBT	This work