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. 2015 Dec 23;10(12):e0145884. doi: 10.1371/journal.pone.0145884

Fig 1. Elimination of SpyCIM1 (ϕ370.4) from its host strain.

Fig 1

A. The prophage-free attB region can be amplified from a SpyCI-free M1 strain MGAS5005 and from SpyCIM1 cured strain CEM1Δ4, but not from the parental SF370SmR strain, where this region is interrupted by the presence of the 13.5 kb chromosomal island in the stationary phase cells. The arrow indicates the size of the expected 1361 bp PCR product following the loss of SpyCIM1. Lane 1 is a no template PCR control. B. Probing of a Southern blot of HindIII digested chromosomal DNA with a mutS-mutL probe showed SF370SmR has an altered pattern due to the presence of the integrated SpyCIM1 chromosome (indicated by the arrow), compared to CEM1Δ4, which has an identical hybridization pattern with SpyCIM1-free strains MGAS5005 and K56. MW: Kilobase molecular weight marker (Life Technologies, Grand Island, NY).