|
Strains
|
Genotype/Relevant characteristics
|
Reference
|
| SF370 |
emm1 (contains prophages ϕSF370.1, ϕSF370.2, ϕSF370.3, and SpyCIM1) |
[1, 14, 15] |
| SF370SmR |
Spontaneous streptomycin resistant derivative of SF370 |
[19] |
| CEM1KRΔ Spy2136
|
SF370SmR ΔSpy2136::(aacA-aphD) / (rpsL
WT
))
|
[19] |
| CEM1Δ4 |
SF370SmRΔSpyCIM1; cured of SpyCIM1 |
[19] |
| MGAS5005 |
emm1 (SpyCI-free M1 strain) |
[21] |
| K56 |
emm12 (SpyCI-free M12 strain) |
[22] |
|
Plasmids
|
Description
|
|
| pFWKR |
Counter-selection vector with janus cassette, (aacA-aphD) / (rpsL
WT
)) between MCS for phage elimination |
[19] |
| pFWKR- Spy2136
|
pFWKR with flanking regions of Spy2136 for inactivation of SpyCIM1 primase gene |
[19] |
|
PCR Primers
|
Sequence
|
Product size
|
| mutSL-L |
5' AATCGCCAGT TCCTGATGTC |
1361 bp |
| mutSL-R |
5' GGGCTGCTGA TGATTTGATT |
|
| int4-L |
5’ GTCGCTGTCT CATTTGATAG AGCTT |
401 bp |
| int4-R |
5’ CCAACAAGGA GTATTGCTAG GGC |
|
|
qRT-PCR Primers
|
Sequence
|
Product size
|
| nga_RT-L |
5' ACGTTAGCCG CAAATACCAC |
89 bp |
| nga_RT-R |
5' GCTTGTAACG TGGGAAGCTC |
|
| slo-RT-F |
5' AAGCTCCGCC ACTCTTTGTGA |
103 bp |
| slo-RT-R |
5' GCACTAAAGG CCGCTTCAAC 3' |
|
| norA_RT-L |
5' AAACGACGAC CAAACACCTC |
102 bp |
| norA_RT-R |
5' TCAGACTAGC CAGGCAGGAT |
|
| emm RT-L |
5' GCAAAACTAA GAGCTGGAAA |
139 bp |
| emm RT-R |
5' TAGTTTCCTT CATTGGTGCT |
|
| speB RT-L |
5' AGCAGTTGCA GTAGCAACACAT |
141 bp |
| speB-RT-R |
5' CTCCTTGATT CAAAAGGCATTC |
|
| hasB_RT-L |
5' TCCTCAAACG CTAATTGAAGC |
92 bp |
| hasB_RT-R |
5' CCCGCTCTTC TAAGACGTTG |
|
| 16S_rRNA-L |
5’ AGCGTTGTCC GGATTTATTG |
126 bp |
| 16S_rRNA-R |
5’ CACTCTCCCC TTCTGCACTC |
|
