Table 5. Putative candidate gene-based markers located on major QTL regions for PMR and FS.
Trait | Gene ID | Marker type a | Primer sequence (5’– 3’) | SNP location [Chr.(bp)] | Enzyme | Product size (bp) b | |
---|---|---|---|---|---|---|---|
AM | TS | ||||||
PMR | Cla019831 | CAPS | F:CTTTTGCTTGCATTGTGCAT | 2(26751854) | TaqI | 527 | 334,223 |
R:GGATGCAAAGGAGCTGTTTC | |||||||
Cla019844 | dCAPS | F:ATTGAAGACCGTCCTGCCTTTCTCATAAAAAGT | 2(26586794) | MseI | 160 | 192 | |
R:GCGCAGCCAAATAATTCAGT | |||||||
FSI | Cla011257 | dCAPS | F:CCTATTTCACCAAACTCTCTCG | 3(26846820) | EcoRI | 345 | 373 |
R:TCCACTAAGACTACTTCTCGATTCCATGAAT |
aCAPS, cleaved amplified polymorphic sequence; and dCAPS, derived-cleaved amplified polymorphic sequence
bAM, ‘Arka Manik’; and TS, ‘TS34’