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. 2015 Dec 23;10(12):e0145321. doi: 10.1371/journal.pone.0145321

Fig 1. Schematic of the hormonal manipulation protocol used in the present study.

Fig 1

Animals (n = 41) were pre-synchronized by intramuscular injection of GnRH agonist and, 7 days later an injection of Prostaglandin F2 alpha (PGF) analog. At this day [day -20 (D-20)] animals received an ESTROTECT Heat detector device and estrus detection was performed twice daily from D-20 to D-10. All animals received a new intravaginal P4-releasing device on D-10 along with an intramuscular injection of 2 mg estradiol benzoate. Simultaneously, cows in the LF-LCL received an intramuscular injection of PGF. The P4 devices were removed on day -2, 42 h and 30 h before the GnRH injection in the LF-LCL and the SF-SCL groups, respectively. All animals received a PGF injection at P4 device removal and a second PGF injection 6 h later. Ovulation was induced by an injection of GnRH agonist on D0.