Table 3. Statistics from low coverage whole genome sequencing and microsatellite discovery.
Species | Genome coverage (X-fold depth) | GC% | # gSSRs | # PALs | gSSRs per Mb | PALs per Mb | % of PALs matching a plant gene | % of trinucleotide PALs matching a plant gene |
---|---|---|---|---|---|---|---|---|
Black cherry | 2.63 | 36.8 | 30,818 | 8,932 | 24.0 | 6.9 | 6.8% | 18.0% |
Black walnut | 1.71 | 34.7 | 44,577 | 12,751 | 37.4 | 10.7 | 2.7% | 5.6% |
Blackgum | 0.04 | 34.0 | 8,154 | 1,103 | 153.8 | 20.8 | 1.4% | 2.5% |
Green ash | 0.77 | 34.7 | 13,590 | 2,650 | 18.1 | 3.5 | 2.4% | 2.5% |
Honeylocust | 1.71 | 31.6 | 30,997 | 4,715 | 14.4 | 2.2 | 2.4% | 5.9% |
Redbay | 1.49 | 37.9 | 56,887 | 18,167 | 30.4 | 9.7 | 1.3% | 1.8% |
Sugar maple | N/A | 38.1 | 6,051 | 891 | 59.0 | 8.7 | 1.3% | 1.9% |
Sweetgum | 0.14 | 36.9 | 7,340 | 1,889 | 63.7 | 16.4 | 1.2% | 1.5% |
White ash | 0.34 | 33.9 | 5,325 | 1,079 | 16.8 | 3.4 | 3.2% | 2.2% |
White oak | 0.11 | 33.6 | 6,995 | 1,005 | 84.0 | 12.1 | 1.6% | 4.8% |
Genome coverage and GC percentages calculated from reconstructed fragments, i.e. overlapping paired ends joined to create a single sequence. Potentially amplifiable loci (PALs) are gSSRs (genomic SSRs) that have flanking primers, allowing them to be tested for polymorphism. The rates of these markers per million bases (Mb) are calculated based on the total bases of reconstructed fragments for each species.