Table 5. Frequency of repeat patterns for two base pair and three base pair motif gSSRs.

For both 2- and 3-base pair repeat motifs, repeats with a lower GC% was more commonly found. Shifted and reverse complement motifs were merged into a single category; e.g. AG shifted by a single base is GA, and on the reverse strand is CT or TC. Abbreviations: BC = black cherry, BW = black walnut, BG = blackgum, GA = green ash, HL = honeylocust, RB = redbay, SM = sugar maple, SG = sweetgum, WA = white ash, WO = white oak.

SSRs with 2-base Repeat Motif	BC	BW	BG	GA	HL	RB	SM	SG	WA	WO
AT|TA	38%	56%	74%	23%	64%	55%	51%	39%	32%	55%
AG|GA|CT|TC	57%	39%	14%	42%	22%	39%	36%	50%	49%	37%
AC|CA|TG|GT	5%	5%	12%	35%	14%	6%	13%	11%	19%	8%
GC|CG	0%	0%	0%	0%	0%	0%	0%	0%	0%	0%
SSRs with 3-base Repeat Motif	BC	BW	BG	GA	HL	RB	SM	SG	WA	WO
AAT|ATA|TAA|ATT|TTA|TAT	52%	59%	78%	53%	64%	37%	51%	38%	65%	58%
ATG|TGA|GAT|CAT|ATC|TCA	10%	5%	3%	8%	1%	14%	8%	4%	8%	7%
AAG|AGA|GAA|CTT|TTC|TCT	19%	26%	13%	22%	21%	32%	27%	46%	14%	23%
AGT|GTA|TAG|ACT|CTA|TAC	1%	1%	0%	3%	0%	2%	0%	0%	2%	1%
AAC|ACA|CAA|GTT|TTG|TGT	5%	2%	3%	5%	11%	6%	7%	6%	3%	9%
CCA|CAC|ACC|TGG|GTG|GGT	3%	1%	0%	6%	0%	2%	2%	2%	5%	2%
AGC|GCA|CAG|GCT|CTG|TGC	4%	2%	1%	1%	1%	2%	1%	0%	1%	0%
AGG|GAG|GGA|CCT|CTC|TCC	5%	4%	3%	2%	1%	4%	2%	3%	2%	0%
ACG|CGA|GAC|CGT|GTC|TCG	0%	0%	0%	0%	0%	0%	0%	0%	0%	0%
GGC|GCG|CGG|GCC|CCG|CGC	0%	0%	0%	0%	1%	0%	0%	0%	0%	0%