Figure 5.

Effect of digitoxin and MonoD in combination with paclitaxel on H460 cancer cells. (A) Concentration-dependent effect of PX alone or in the presence of Dig (20 nM) or MonoD (20 nM). Cell viability was measured by the MTT assay after treatment for 48 or 72 h. (B) Left panel, representative digital images showing colonies produced by H460 cells following plating of 200 cells. Cells were treated with Dig (20 nM), MonoD (20 nM) or PX (10 nM) alone or in combination (PX 10 nM) + Dig (20 nM) or MonoD (20 nM) for 72 h in and then allowed to form colonies for 10 days. C, Control cells were treated with DMSO alone. Right, quantification of the colony forming assay. ^**P<0.05, significantly different between PX 10 nM vs. the combined treatments. PX, paclitaxel; Dig, digitoxin.