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Sequential treatment: single drug → co-treatment (A and b) or single drug → single drug (C and D). Cells were incubated for 24 h with drugs alone at the indicated concentration for 24 h followed by incubation with the indicated combination or drug alone for 48 h. The symbol '–>' indicates the sequence in which the drugs were added after 24 h. Control cells (DMSO) were incubated with DMSO that was also included at equivalent concentrations for all treatments. *P<0.001 and **P<0.05.
