Table 1. Summary of plasmid constructs and primer extension results from E. coli JW2501-1 harbouring the plasmids.

Plasmid	A2503 stop	Remarks on gene constructs
pBRCfr	+++	codon optimized cfr gene
pBRCfrRlmN	-	approximately half cfr gene + half rlmN gene
pBRRlmNCfr	-	approximately half rlmN gene + half cfr gene
pBRCfr1234567rRlmN	-	cfr gene with regions 1–7 of rlmN gene
pBRCfr34567rRlmN	-	cfr gene with regions 3–7 of rlmN gen
pBRCfr12rRlmN	-	cfr gene with regions 1 and 2 of rlmN gene
pBRCfr234567rRlmN	-	cfr gene with regions 2–7 of rlmN gene
pBRCfr1XrRlmN	-	cfr gene with region 1 of rlmN gene
pBRCfr2XrRlmN	(+)	cfr gene with region 2 of rlmN gene
pBRCfr3XrRlmN	++	cfr gene with region 3 of rlmN gene
pBRCfr4XrRlmN	+	cfr gene with region 4 of rlmN gene
pBRCfr5XrRlmN	-	cfr gene with region 5 of rlmN gene
pBRCfr6XrRlmN	-	cfr gene with region 6 of rlmN gene
pBRCfr7XrRlmN	(+)	cfr gene with region 7 of rlmN gene
pBRRlmN1234567rCfr	-	rlmN gene with regions 1–7 of cfr gene
pBRRlmN34567rCfr	-	rlmN gene with regions 3–7 of cfr gene
pBRRlmN12rCfr	-	rlmN gene with regions 1 and 2 of cfr gene
pBRRlmN	+	rlmN gene
pBR322	-	Parent plasmid
No plasmid	-	Host control

The strength of the modification stop on gels from the primer extension was visually assessed and is indicated by 1–3 plusses. (+) Ambiguous band, - no band present.