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. 2015 Dec 23;10(12):e0145009. doi: 10.1371/journal.pone.0145009

Fig 3. The promoter activity of creD gene was regulated by the bacterial culture density.

Fig 3

(A) The impact of plasmid and tetracycline on the bacterial growth and C23O expression of strain KJCreD23. Plasmid pRK415 was transported into KJCreD23 by conjugation. The bacterial growth (by recording the OD450nm) and C23O activity expressed from KJCreD23, KJCreD23(pRK415), and KJCreD23(pRK415) with 30 μg/ml tetracycline were monitored every 3 h. (B) The impact of kanamycin (Km) on the bacterial growth and C23O expression of strain KJΔBCCreD23. The bacterial growth (by recording the OD450nm) and C23O activity of KJΔBCCreD23 in the absence and presence of kanamycin (1 or 5 μg/ml) were monitored every 3 h. (C) The impact of menadione (K3) on the bacterial growth and C23O expression of strain KJΔBCCreD23. The bacterial growth (by recording the OD450nm) and C23O activity of KJΔBCCreD23 in the absence and presence of K3 (2 or 30 μg/ml) were monitored every 3 h. (D) The impact of benzalkonium chloride (BC) on the bacterial growth and C23O expression of strain KJΔBCCreD23. The bacterial growth (by recording the OD450nm) and C23O activity of KJΔBCCreD23 in the absence and presence of BC (1 or 5 μg/ml) were monitored every 3 h.