Fig 4. The impact of phosphor-mimic variant of CreB, CreB(D55E), on the promoter activity of creD gene.

Overnight culture of strain KJCreD23Fua::CreB(D55E) was inoculated into the fresh medium to the OD₄₅₀ of 0.15 in the absence and presence of fusaric acid (30 μg/ml). Cells were grown aerobically and the C23O activity were measured every 3 h.