. 2015 Dec 14;10(12):e0144729. doi: 10.1371/journal.pone.0144729

Table 2. The Salmonella bioassay of the irrigative wastewater taken from the Dongming discharging river in Shijiazhuang city (n = 6).

	TA97 (-S9)		TA97 (+S9)		TA98 (-S9)		TA98 (+S9)
Dose (μl/plate)	MI ^a	Revertant colonies	MI ^a	Revertant colonies	MI ^a	Revertant colonies	MI ^a	Revertant colonies
NC ^b	1	97.2±10.2	1	100.7±14.7	1	30.2±2.3	1	30.5±1.4
25	1.2	116.0±15.7	1.1	111.2±13.2	2.3 ^d	67.5±7.3^*	3.0 ^d	90.0±14.4^*
50	1.2	119.7±15.6	1.2	121.8±7.0	3.2 ^d	97.2±4.1^*	3.4 ^d	102.3±12.4^*
75	1.2	118.0±10.3	1.3	126.8±5.3	3.7 ^d	111.7±7.6^*	4.5 ^d	137.3±4.7^*
100	1.3	129.0±6.4	1.3	130.0±7.2	5.0 ^d	150.0±7.2^*	5.3 ^d	163.2±9.3^*
PC	21.5 ^d	2078.5±118.0	12.9 ^d	1274.5±108.8	98.2 ^d	2950.7±83.3	85.9 ^d	2618.5±268.1
	TA100 (-S9)		TA100 (+S9)		TA102 (-S9)		TA102 (+S9)
Dose (μl/plate)	MI ^a	Revertant colonies	MI ^a	Revertant colonies	MI ^a	Revertant colonies	MI ^a	Revertant colonies
NC ^b	1	116.3±6.6	1	120.0±7.3	1	221.3±11.9	1	220.0±8.8
25	1.1	132.3±10.3	1.1	134.8±8.0	1.1	238.3±11.6	1.1	242.3±12.7
50	1.1	130.0±4.7	1.1	133.5±11.0	1.1	250.8±7.8	1.2	263.8±10.0
75	1.1	130.0±7.1	1.2	144.8±13.7	1.2	258.3±12.0	1.2	263.5±11.0
100	1.2	139.3±5.9	1.1	131.3±8.8	1.1	242.7±7.4	1.1	242.7±7.4
PC ^c	20.3 ^d	2368.3±206.9	12.4 ^d	1475.5±41.5	9.1 ^d	2005.3±120.7	5.3 ^d	1158.3±163.0

^a: Mutagenic index (MI): number of revertant colonies induced in the sample/number of spontaneous revertant colonies in the negative control.

^b: Negative control (NC) for all strains: sterile distilled water

^c: Positive controls (PC) in experiments: Without S9: 0.2 μg/plate 9-fluorenone was used for strain TA97 and TA98, 2.5 μg/plate sodium azide for strain TA100, 30 μl/plate methylsulfonic methylester was used for strain TA102; With S9: 10 μg/plate 2-aminoflurene was used for strain TA97, TA98, and TA100; 50 μg/plate 1,8-dihydroxyanthraquinone was used for strain TA102

^d: MI was more than twice that of the negative control.

*: Significant dose-response relationships were observed