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. Author manuscript; available in PMC: 2017 Jan 11.
Published in final edited form as: Chem Commun (Camb). 2015 Nov 5;52(3):553–556. doi: 10.1039/c5cc08106h

Figure 3.

Figure 3

Western blot analysis of azido-BSA – Biotin-azide conjugation using different protein loading: lanes 1–5, 2 µg per lane and lanes 6–7, 2 µg per lane. Lanes 2,7: photo-DIBOD 1a, azido-BSA and Biotin-azide irradiated; Lanes 3,8: 2a, azido-BSA, and Biotin-azide incubated overnight; Lanes 4,9: photo-DIBOD 1a, azido-BSA, and Biotin-azide incubated overnight in the dark; Lane 5,10: azido-BSA and Biotin-azide incubated overnight.