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. 2015 Oct 31;90:117–126. doi: 10.1007/s11103-015-0400-4

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© The Author(s) 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 2 — Localisation of GLDH by BN-PAGE. Mitochondrial complexes of Col-0 and the complex I mutant ndufs4 were resolved by Blue-Native PAGE. Duplicate gels were run and either stained with Coomassie (left panel) or transferred onto a membrane for western blot analysis with anti-GLDH (middle panel) or anti-CA (right panel) antibodies. The position of selected respiratory complexes is indicated on the left. The size (in kDa) of complex I and the different assembly intermediate of complex I is indicated on the right