Fig. 3.

Localisation of GLDH by two-dimensional BN-SDS-PAGE. Mitochondrial complexes of Col-0 and the complex I mutant ndufs4 were resolved by BN-SDS-PAGE. Duplicate gels were run and either stained with Coomassie (top panels) or transferred onto a membrane for western blot analysis with anti-GLDH (middle panels) or anti-CA (bottom panels) antibodies. The positions of complexes I, III and V of the OXPHOS system are indicated above the top panel. The positions of complex I and its assembly intermediates are indicated above of the central panels. Complexes not detected in the western blots are indicated in grey to facilitate comparison between the two samples