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. 2015 Dec 24;6:1117. doi: 10.3389/fpls.2015.01117

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Copyright © 2015 Ramalingam, Kudapa, Pazhamala, Garg and Varshney.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Confirmation of PPI strength using qRT-PCR. The qRT-PCR analysis quantified the expression of two reporter genes HIS3 and ADE2 for determining the strength of PPI for the selected yeast colonies (PPI-1, PPI-4, and PPI-5). These genes are under the control of Gal4-responsive promoters. The interaction between the bait and prey proteins allow the Gal4-responsive HIS3 and ADE2 genes to biosynthesis histidine and adenine for cell growth.