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Immunodetection of xylose catabolic proteins. Protein samples extracted from Synechocystis transformant strains, X-Ut1, X-Ut2, X-Ut3, and X-Ut4 and the WT strain as the negative control were probed with specific polyclonal antibodies for the XylA (D-xylose isomerase) and XylB (D-xylulokinase) enzymes. Each lane was loaded with 20 μg of total protein fraction. The expected molecular masses for XylA and XylB proteins are indicated.
