Table 2.
Frequency of micronucleated lymphocytes. Number of micronuclei observed perslide (MN) and number of mononucleated (N 1), binucleated (N 2), and polynucleated (N p) lymphocytes and anaphase bridges (AB) observed pergroup. Based on these values, the micronucleated formation frequency (MNr0) and the cytokinesis-block proliferation index (CBPI) and the media () are shown.
Sample | MN | N 1 | N 2 | N p | AB | MNr0 | CBPI |
---|---|---|---|---|---|---|---|
Negative control | |||||||
01 | 7 | 954 | 22 | 24 | 3 | 0.3181 | 1.070 |
02 | 5 | 963 | 19 | 18 | 3 | 0.2631 | 1.055 |
03 | 6 | 949 | 22 | 29 | 0 | 0.2727 | 1.051 |
04 | 3 | 953 | 17 | 30 | 1 | 0.1764 | 1.077 |
05 | 7 | 960 | 22 | 18 | 5 | 0.3181 | 1.058 |
Total | 28 | 4,779 | 102 | 119 | 12 | ||
| |||||||
Positive control (cyclophosphamide) | |||||||
01 | 32 | 906 | 51 | 43 | 6 | 0.6274 | 1.137 |
02 | 31 | 873 | 62 | 65 | 8 | 0.5000 | 1.192 |
03 | 48 | 890 | 70 | 40 | 7 | 0.6857 | 1.150 |
04 | 54 | 903 | 62 | 35 | 14 | 0.8709 | 1.132 |
05 | 50 | 875 | 52 | 73 | 4 | 0.9615 | 1.198 |
Total | 215 | 4,447 | 297 | 256 | 39 | ||
| |||||||
BPV-1 E6 recombinant oncoprotein | |||||||
01 | 51 | 892 | 60 | 48 | 4 | 0.8500 | 1.156 |
02 | 50 | 894 | 74 | 32 | 9 | 0.6756 | 1.138 |
03 | 49 | 900 | 53 | 47 | 10 | 0.9245 | 1.147 |
04 | 40 | 901 | 41 | 58 | 10 | 0.9756 | 1.157 |
05 | 38 | 905 | 43 | 52 | 10 | 0.7307 | 1.147 |
Total | 228 | 4,492 | 271 | 237 | 43 |