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. 2015 Dec 10;2015:263630. doi: 10.1155/2015/263630

Figure 5.

Figure 5

Effects of BA on IFN-γ production in human PBMC. A/PR/8/34 infected PBMC were treated with gradient concentration of BA at 0 μM, 0.1 μM, 1 μM, 10 μM, 100 μM, and 1 mM for 48 h at 37°C. (a) Total RNA was isolated from PBMC and subjected to RT-PCR. The RT-PCR of human IFN-γ products was analyzed on 1% agarose gel electrophoresis. (b) The cytosol proteins were obtained from PBMC and analyzed by Western Blotting with anti-IFN-γ antibody. (c) IFN-γ production in cell culture supernatant infected with (c) or without (d) A/PR/8/34 virus infection was calculated by ELISA, and the results were determined from a standard curve.  ∗Levels of significance of P < 0.05,  ∗∗levels of significance of P < 0.01.