Figure 7.

Effects of BA on IFN-γ production in human CD4⁺ and CD8⁺ T cells and NK cells. Human PBMC sorted CD4⁺ and CD8⁺ T cells and NK cells were treated with or without 1 mM BA for 24 h, 48 h, and 72 h. (a) Total RNA was isolated from CD4⁺ and CD8⁺ T cells and NK cells and subjected to RT-PCR. The RT-PCR products of human IFN-γ were analyzed on 1% agarose gel electrophoresis. (b) The cytosol protein was obtained and analyzed by Western Blotting using anti-human IFN-γ antibody. (c) Cell culture supernatants were obtained and analyzed by ELISA; results were expressed as the sample concentrations, determined from a standard curve. Each data shows mean ± SE of three respective determinations.  ^∗∗Levels of significance of P < 0.01 against untreated cells.