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. 2015 Dec 17;7(3):6246. doi: 10.4081/dr.2015.6246

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©Copyright L. Rottboell et al.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Valrubicin’s effect on P. acnes-induced mRNA and protein expression levels of inflammatory cytokines by NHEKs. NHEKs from four healthy donors, were cultured in the presence of freshly prepared P. acnes. The effect of valrubicin on P. acnes-induced mRNA expression levels of IL-1β (a), IL-8 (b), and TNF-α (c) was evaluated after 2 hrs of stimulation. mRNA expression levels were normalized to the expression levels of the housekeeping gene, ribosomal protein large P0 (RPLP0) and to the untreated control (no P. acnes bacteria added). The effect of valrubicin on P. acnes-induced protein expression levels of IL-1β (d), IL-8 (e), and TNF-α (f) was evaluated after 12 hrs of stimulation. Protein expression levels were determined by ELISA and normalized to the untreated control (no P. acnes bacteria added). Values are presented as mean + standard deviation (NHEKs, n=4 donors, cultured in triplicate).