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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Talanta. 2015 Jun 20;146:839–843. doi: 10.1016/j.talanta.2015.06.003

Figure 4.

Figure 4

Base peak electropherograms in triplicate runs (A – full run. B – closeup) and parent ion mass spectrum on intact antibody (C). For A and B, data are treated with a Lowess filter with Gaussian kernel and 10 point span. NL is the normalization level, which is the maximum value of the filtered electropherogram. For C, the parent ion mass spectra from 29.6 to 30.7 minutes were resampled at a common mass axis with 20,000 points between 1,000 and 4,000 Da. The spectra were then summed, the summed spectrum was treated with a Lowess filter with Gaussian kernel and 20-point span, and the averaged spectrum was normalized to unit height. Experimental conditions: BGE: 5% acetic acid, Separation voltage: 250 v/cm, sample matrix: 10 mM NH4HCO3(pH 8), sample injection: 500 mbar for 0.3 min