Figure 2.

Assessing the coverage of the transcriptomes for the four life cycle/all stages with random subsampling of the original raw reads. (A) Changes in the number of contigs with significant BLAST hits as sample size is increased by an increment of 12.5% from 12.5% to 100% of the original raw reads; (B) Changes in N50 for these same samples of the original raw reads. In Riesgo et al. [33], subsamples of increasing size were generated by adding an increment of 5 million more reads to the previous subset. Thus, our subsamples are more independent than theirs in that each represents its own separate draw of the original raw reads for a stage.