Table 3.
Gene ontology terms with significantly up- or down-regulated gene groups during the two life cycle transitions. These significant GO terms are for the subsets of 1025 and 716 annotated contigs in Figure 5A with a ≥4-fold expression change during the pre-competent larva/post-larva and post-larva/adult transitions, respectively. Enrichment tests of the GO terms are performed for the two life cycle transitions with the reference distribution for all stages and with Fisher’s exact test after correcting the significance level (α = 0.05) for the false discovery rate with the Benjamini-Hochberg [79] method.
| Pre-Competent Larva/Post-larva Transition | Post-Larva/Adult Transition | ||
|---|---|---|---|
| Up-Regulated GO Terms (32) | Down-Regulated GO Terms (17) | Up-Regulated GO Terms (4) | Down-Regulated GO Terms (27) |
| Actin binding {F:GO:0003779} | Cellular macromolecule biosynthetic process {P:GO:0034645} | Hydrolase activity {F:GO:0016787} | Cell-cell junction {C:GO:0005911} |
| Axonemal dynein complex {C:GO:0005858} | Cellular protein metabolic process {P:GO:0044267} | Peptidase regulator activity {F:GO:0061134} | Cellular protein metabolic process {P:GO:0044267} |
| Branching morphogenesis of an epithelial tube {P:GO:0048754}* | Golgi membrane {C:GO:0000139} | Peroxidase activity {F:GO:0004601} | Cytoplasm {C:GO:0005737} |
| Calcium-dependent protein binding {F:GO:0048306} | Mitochondrial matrix {C:GO:0005759} | Phenanthrene-epoxide hydrolase activity {F:GO:0019118} | Cytoplasmic transport {P:GO:0016482} |
| Cell morphogenesis {P:GO:0000902}* | mRNA splicing, via spliceosome {P:GO:0000398} | Cytosol {C:GO:0005829} | |
| Cilium movement {P:GO:0003341} | Multi-organism metabolic process {P:GO:0044033} | Endomembrane system organization {P:GO:0010256} | |
| Cilium or flagellum-dependent cell motility {P:GO:0001539} | ncRNA metabolic process {P:GO:0034660} | Establishment of vesicle localization {P:GO:0051650} | |
| Cytoplasmic vesicle part {C:GO:0044433} | Nucleoplasm part {C:GO:0044451} | Exocytosis {P:GO:0006887} | |
| Epithelial cell differentiation {P:GO:0030855}* | Positive regulation of stress-activated MAPK cascade {P:GO:0032874} | Golgi vesicle transport {P:GO:0048193} | |
| Establishment of planar polarity {P:GO:0001736} | Ribonucleoprotein complex {C:GO:0030529} | Homeostatic process {P:GO:0042592} | |
| Extracellular space {C:GO:0005615} | Ribonucleoprotein complex biogenesis {P:GO:0022613} | Macromolecular complex {C:GO:0032991} | |
| Imaginal disc development {P:GO:0007444}* | Ribonucleoprotein complex subunit organization {P:GO:0071826} | Macromolecular complex subunit organization {P:GO:0043933} | |
| Inner ear development {P:GO:0048839}* | RNA binding {F:GO:0003723} | Macromolecule modification {P:GO:0043412} | |
| Lateral ventricle development {P:GO:0021670}* | RNA modification {P:GO:0009451} | Nucleoplasm {C:GO:0005654} | |
| Left/right axis specification {P:GO:0070986} | RNA splicing {P:GO:0008380} | Nucleotide binding {F:GO:0000166} | |
| Locomotion {P:GO:0040011} | Single-organism metabolic process {P:GO:0044710} | Nucleus {C:GO:0005634} | |
| Microtubule motor activity {F:GO:0003777} | Viral life cycle {P:GO:0019058} | Organic substance transport {P:GO:0071702} | |
| Microvillus {C:GO:0005902} | Posttranscriptional regulation of gene expression {P:GO:0010608} | ||
| Neurological system process {P:GO:0050877} | Purine ribonucleoside binding {F:GO:0032550} | ||
| Neuron projection {C:GO:0043005} | Purine ribonucleoside triphosphate binding {F:GO:0035639} | ||
| Neuron projection development {P:GO:0031175}* | Receptor-mediated endocytosis {P:GO:0006898} | ||
| Peptidase activity {F:GO:0008233} | RNA binding {F:GO:0003723} | ||
| Peroxidase activity {F:GO:0004601} | RNA processing {P:GO:0006396} | ||
| Phagocytic vesicle membrane {C:GO:0030670} | Single-organism cellular localization {P:GO:1902580} | ||
| Protein heterotetramerization {P:GO:0051290} | Single-organism membrane organization {P:GO:0044802} | ||
| Receptor activity {F:GO:0004872} | Single-organism organelle organization {P:GO:1902589} | ||
| Regulation of anatomical structure morphogenesis {P:GO:0022603}* | Small molecule catabolic process {P:GO:0044282} | ||
| Serine-type peptidase activity {F:GO:0008236} | |||
| Signal transducer activity {F:GO:0004871} | |||
| Sperm principal piece {C:GO:0097228} | |||
| Synaptic cleft {C:GO:0043083} | |||
( ) = total counts of significant GO terms; { } = GO identification numbers; * = the eight GO terms with “morphogenesis”, “differentiation”, and “development” whose gene groups are significantly up-regulated during the pre-competent larva/post-larva transition (see text).